Kirish: Tibbiy tadqiqotlarning ajralmas bo'g'ini, ya'ni hayvonlar tajribalari mavjud. To'liq bo'lmagan statistik ma'lumotlarga ko'ra, har yili butun dunyoda millionlab hayvonlar ilmiy tajribalar qurboni bo'lishadi. Bu qonli bo'lib tuyulsa-da, bu tibbiyotning rivojlanishi ham o'tishi kerak bo'lgan jarayondir. Biroq, so'nggi yillarda 3D bioprinting texnologiyasining rivojlanishi bilan, ba'zi bioprinted konstruktsiyalar tirik to'qimalarni funktsional almashtirishga erishish va asta-sekin hayvonlarning tajribalarini almashtirish maqsadiga erishishi kutilmoqda.
△In 2014, about 400,000 mice died in the laboratory
2022-yil 18-fevral, Yevropa Ittifoqi (EI){2}}moliyalash loyihasi 3D bioprinting orqali hayvonlarni eksperimental tibbiy tadqiqotlarda sinovdan o‘tkazishni qisqartirmoqchi. Kataloniya Bioinjeneriya Instituti (IBEC) tomonidan muvofiqlashtirilgan BRIGHTER (Fotolitografiya orqali bioprinting: Yuqori aniqlik va tezlikda murakkab to‘qimalar muhandisligi) loyihasi to‘qimalar muhandisligi va regenerativ tibbiyotda taxidermiyadan foydalanishni kamaytirish uchun 3D bioprinting jarayonlari uchun yangi yondashuvlarni ishlab chiqmoqda. bu hududlar. Alohida ta'kidlash joizki, loyiha naqshli lazerli yorug'lik varaqlari asosida yangi bioprinting texnikasi yordamida inson terisini ishlab chiqarishga qaratilgan.
Professor Elena Martinez, coordinator of the BRIGHTER project, said: "Our innovative 3D bioprinting system not only achieves tissue closer to the real thing, but is also much faster than current systems, an essential factor in ensuring the viability of new tissue."
△ A small square containing a matrix of skin cells. Photo via IBEC.
3D bosib chiqarish yordamida hayvonlarni sinovdan o'tkazishni qisqartirish
3D bioprinting texnologiyasi soʻnggi oʻn yil ichida sakrash va chegaralarda rivojlandi va bemorning oʻziga xos toʻqimalarini -ishlab chiqishda katta yutuqlarga erishdi. Ushbu ishlanmalar kelajakda samaradorlik sinovlari uchun va'da bergan bo'lsa-da, to'qimalar hali ham asosan eksperimentaldir va odamlarda dori sinovlari o'nlab yillar davom etadi. Shu bilan birga, akademik doiralar ham, sanoat ham buni o'zgartirish ustida ishlamoqda, shved bioprinter ishlab chiqaruvchisi CELLINK hayvonlar uchun zararsiz hujayralar sinovi modellari bo'yicha tadqiqotlarini ilgari surish va Shtutgart universitetida saraton dori-darmonlarining samaradorligini yo'q qilish uchun miniatyura teri modellaridan foydalanishga va'da berdi. hayvonlar sinovi.
Elsewhere, Fluicell's Biopixlar platform has produced highly complex neural models that show potential for future clinical drug screening applications, while UpNano's NanoOne Bio system is focusing on the fabrication of cell culture microstructures that may have Helps reduce the number of animal experiments behind clinical trials.
△CELLINK has acquired in vitro technology specialist MatTek to create a harmless drug testing model. Photo via MatTek.
Hayvonlarni sinovdan o'tkazish uchun ko'proq insoniy muqobil
In addition to IBEC, the Goethe University Frankfurt, the Technion Center in Israel and the biotechnology companies Mycronic and Cellendes are also participating in the BRIGHTER project. The program hopes to overcome many of the technical barriers that currently limit the fabrication of complex human tissue. The partners are collaborating on the development of a novel light-sheet bioprinting process capable of producing complex and accurate in vitro models that can be used for cosmetic and drug testing in the pharmaceutical industry and research settings. To fine-tune the technology, the BRIGHTER team is working to 3D print human skin, a highly complex tissue composed of multiple cell types and structures, such as sweat glands and hair follicles. Hydrogels will form a key component of the bioprinting process, as they form the basis for cells to grow and form new tissues, and they can also be personalized using a patient's own cells. To print skin with the desired structure, shape, and consistency, the researchers are using advanced imaging techniques that combine illumination from light sheets and high-resolution digital masks. By applying the laser directly to the hydrogel, the cells within it can be "patterned" and shaped into the right shape, allowing the team to control the stiffness, shape and size of the 3D printed structures.
The ability to shape hydrogels at a high level is especially critical for successfully printing human skin, because this tissue is made up of many layers of cells of different types. According to the BRIGHTER team, their bioprinting process was also able to create the blood vessels of the printed tissue and enable the function of sebaceous and sweat glands, as well as hair follicles to grow hair. Dr Nuria Torras, a postdoctoral researcher at IBEC, said: "We hope to be able to print a skin sample with an area of 1 square centimeter and a thickness of 1 mm in about 10 minutes with a cell viability rate of over 95 percent , greatly improving current bioprinting conditions. "The BRIGHTER project hopes that successful printing of the in vitro skin model will validate its potential for use in pharmaceutical and research settings, and ultimately reduce animal testing for drug and cosmetic testing.
Guangmai Technology sog'lom va aqlli yorug'lik manbalari bilan chuqur shug'ullanadi, bozorga UVA UVB UVC LED, infraqizil IR LED VCSEL mahsulotlari va echimlarini taqdim etadi. Sog'lom va aqlli hayotni yaratish uchun yorug'lik texnologiyalaridan foydalanishni birgalikda targ'ib qilish uchun ichki va tashqi bozorlarda yuzlab yuqori sifatli-hamkorlarga ega. .
